It is in this panel of the Data Entry Wizard that you enter the data. Because you selected Sample Data as the Data Entry Method, sample data appears as the first series.

Notice how [Substrate] and [Malonate] appear as the independent variables.

The first column, [S] in this example, is the first independent variable: the substrate Succinate.

Because you selected [Substrate], this column of substrate concentrations will appear in each subsequent series.

The second column, [I], is the second independent variable, the inhibitor Malonic Acid.

The inhibitor Malonic Acid appears as the constant independent variable in each series.

The remaining columns represent the Dependent Variable (Rate). Columns V1, V2 and V3 represent the Dependent Variables. In this example, there are three repeated rate or velocity measurements or replicates. The Data Entry Wizard can accommodate up to 16 replicates.

You also can switch the constant independent variable.

Select [Malonate].

Notice the values switch between column one and two. [Succinate] is held constant.

Select [Succinate] again, and then click Next.

Now you can enter another series of data.

But first, let's take a look at how the data you've just entered appears in a SigmaPlot worksheet.

On the menus click:

Window
EK Data 1

Here is how a worksheet appears with Enzyme Kinetics data.

Notice how the options you set earlier in the Data Entry Wizard appear in the worksheet.

And that so far, you have entered one data series.

The Dependent Variables (Rate) appear here.

Now let's go back to the Data Entry Wizard to enter the next series of data.

Of course, because you selected Sample Data as the Data Entry Method, a new data series already appears in the Data Entry Wizard.

Click Next until you reach Series 4, and then click Finish.

Now that you've entered the data, you'll use the Enzyme Kinetics Wizard to analyze it.

Entering Enzyme Kinetics Data