Welcome to the Enzyme Kinetics Tutorial. This tutorial is designed to help you quickly learn how to create Enzyme Kinetics graphs and reports using the Enzyme Kinetics Module.

The SigmaPlot Enzyme Kinetics module streamlines and automates enzyme kinetics analyses. To use the Enzyme Kinetics module, all you have to do is enter your data, and select your analysis options. Once you complete these tasks, the Enzyme Kinetics module automatically analyzes your data and produces the desired statistical reports and graphs.

The Enzyme Kinetics module uses SigmaPlot as the engine for all curve fitting, graphing, and report generation, producing results that you can publish. This tutorial takes you through one of the most common types of analysis: Single Substrate - Single Inhibitor. You will use the Enzyme Kinetics Module to fit sample data to Competitive (Full) and Uncompetitive (Full) models and produce both a Michaelis-Menten and Lineweaver-Burk graph.

Use SigmaPlot's Enzyme Kinetics Module to analyze and graph enzyme kinetics data quickly and easily. Import or enter your data using the Data Entry Wizard, select the type of study and the Enzyme Kinetics Wizard does the rest. You'll get a detailed report of each model you fit and a comparison of the different models to identify the best one for your data, plus interactive graphs that allow you to clearly display your results.

Introduction